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Review Papers | Microbiology | India | Volume 13 Issue 1, January 2024 | Rating: 4.6 / 10
Unraveling the History and Mysteries of RNA Sequencing Technology
Amitesh Chakraborty, Rishav Kar
Abstract: RNA sequencing is a technology used extensively in transcriptomics studies. Next - generation sequencing is incorporated in the RNA - seq approach for RNA profiling to deliver unparalleled resolution for analysing and contrasting gene expression patterns. Data pre - processing is one of three main sections of the RNA - sequencing data analysis workflow. An overview of the techniques used in bulk RNA - seq and single - cell analysis is presented here, with an emphasis on alternative splicing and active RNA production study. Removal of adapters, quality checking, trimming, and filtering are essential components of data pre - processing. The data are then post - processed and subjected to a variety of analyses, such as alternative splicing, differential gene expression, and analysis of active synthesis.
Keywords: RNA - Sequencing, Nascent mRNA analysis, Transcriptomic data analysis, Alternative splicing
Edition: Volume 13 Issue 1, January 2024,
Pages: 1057 - 1065