Genotoxicity Screening of the Crude Ethanolic Extract of Artocarpus heterophyllus (langka) Unripe Fruit Using Allium cepa (Onion) Assay

Abstract: The crude ethanolic extract of Artocarpus heterophyllus (langka) unripe fruit was found out to lower blood sugar in alloxan-induced hyperglycemic white mice (Mus musculus). This study was conducted to determine the genotoxicity (mitotic index (MI) and percent chromosomal aberration ( %CA) of the crude ethanolic extract of A. heterophyllus unripe fruit using A. cepa (onion) assay. Onions with 1.5-2.0cm length of the roots were grown in different concentrations of A. heterophyllus extract. The 3 roots with a length of 2mm from the tips were harvested and fixed. Squash preparations were made for each of the concentration, positive and negative control in 3 replicates and 3 trials. Results showed that 15mg (MI=0.782+0.022, %CA=0.06+0.003) and 20mg (MI= 0.754+0.017, %CA=0.04+0.019) of the crude ethanolic extract per ml of ml of distilled water did not show genotoxicity based on the mitotic index (MI) and %chromosomal aberration (CA). They were comparable to the negative control used (dist. water) (MI=0.756+0.028, CA=0.08+0.022) (P

Keywords: genotoxicity, Artocarpus heterophyllus, Allium cepa assay, mitotic index

Edition: Volume 7 Issue 5, May 2018,

Pages: 388 - 397