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Research Paper | Microbiology | Ivory Coast | Volume 7 Issue 5, May 2018
In vitro Evaluation of the Antibacterial Activity of Extracts of Phyllanthus amarus on Three Germs (Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) of Superinfection of Buruli Ulcer in Cote dIvoire
Bakary Coulibaly [3] | Ibourahema Coulibaly [5] | Kouassi Kouassi Clement [2] | Sylvia Monthaut | Joseph Allico Djaman
Abstract: Complications of Burulis ulcer associated with bacterial superinfections make it difficult to manage this infection. In addition, the rising costs of treating infections caused by resistant bacteria call for alternative care alternatives. The development of new antibacterial molecules is essential to fight against these scourges. It is for this purpose that this study was carried out by evaluating the activity of aqueous and ethanolic extracts of P. amarus on the in vitro growth of three Buruli ulcer superinfection organisms in Ivory Coast. The methods of diffusion in agar medium and in liquid medium were used for the sensitivity test and the determination of the MIC and the CMB. The different tests were performed on three isolated strains of patients with Buruli ulcer. The minimum inhibitory concentrations of the extracts vary between 5 mg / ml and 20 mg / ml and the minimum bactericidal concentrations between 10 mg / ml and 40 mg / ml. The lowest value of MIC and CMB was observed with S. aureus637 while the highest value of these same parameters was obtained on E. coli 250. These extracts exerted a bactericidal activity on P. aeruginosa, S. aureus, E. coli. This could justify the use of this plant in the treatment of various infections in traditional environments.
Keywords: M ulcerans, E coli, P aeruginosa, S aureus, P amarus, antibacterial activity, aqueous extract, ethanolic extract, MIC, CMB, Buruli ulcer,
Edition: Volume 7 Issue 5, May 2018,
Pages: 658 - 661
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