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Research Paper | Microbiology | Egypt | Volume 5 Issue 11, November 2016 | Popularity: 6.5 / 10

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Antimicrobial Activity and Fingerprint Patterns of Some Pathogenic Bacterial Species against Extracted Oils from Genetically Modified Mentha piperita L.

Walid. W. Mohamed, Ekbal. H. Abed El Hafez, Sherin M.A. Sharaf

Abstract: Gamma rays was used in the irradiation process with 2, 4, 6, 8 and 10 K-Rad dose for Mentha piperita L. seeds, Then Essential oils were obtained by hydro-distillation method and chemical composition was analyzed using gas chromatography (Gc) and gas chromatography mass spectrometry (GC/MS). Twelve components were identified throughout the extract and the major represented components were Menthol, Menthone and 1, 8-cineol. A study of antibacterial activity for the extracted essential oils was done on a six pathogenic human bac GC/MS terial species using agar well diffusion method. The minimum inhibitory concentration (MIC) was also done for the six bacterial species. Random Amplified Polymorphic DNA (RAPD) fingerprinting was used to illustrate the antibacterial activity within the bacterial species. The most antibacterial effect of the extracted oils from the irradiated seeds was expressed in dose 10 KR at concentration of 11 for Proteus mirabilis and Klebsiella pneumonia.

Keywords: Mentha piperita L, GC/MS, MIC and RAPD

Edition: Volume 5 Issue 11, November 2016

Pages: 1082 - 1087

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