International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064




Downloads: 118 | Views: 230

Research Paper | Environmental Science Studies | Pakistan | Volume 5 Issue 10, October 2016 | Rating: 6.6 / 10


Effect of Boiling on Removing of Botulinum Toxins from Drinking Water Samples

M Badar [2] | Fatima Batool [2] | Muhammad Idrees [2] | Hafiz Reehan Iqbal [2] | M Ahsan Zia [2]


Abstract: C. Botulinum growing the spores is found all over the world in the samples of soil and marine sediments and a normal healthy person can consume the spores. But when food is contaminated and the spores grow in anaerobic conditions then toxin is created with highly poisonous and toxic when ingested it. In food-borne botulism cases are linked with home conserved meats as fish and vegetables. The conventional methods of chemical treatment of drinking water are included as disinfections, chemical coagulation treatment, heat pasteurization, and filtration. These were very useful drinking water treatment methods but this time needed how to apply this conventional method on present complex chemical contamination situation of drinking water sources. In the present study, the enumeration of C. Botulinum as bacterial specious detected on different media but their related metabolites toxins like botulinum was tested by ELISA method. In this study a new bacterial pathogen was isolated and identified from water samples tested for example C. Botulinum. Toxins of botulinum is seen with very high concentration as 10.5 mg/l in the canal and water storage tanks due to high microbial growth detected by simple conventional method of media culture. Boiling water with chemical treatment give maximum reduction as 99 % of toxins level of different sample which very useful for domestic level. Increasing the toxins level in any living organism can create the chronic and acute diseases which is both harmful for society and economics.


Keywords: Toxins, Boiling Water, Microcystins, Coagulation


Edition: Volume 5 Issue 10, October 2016,


Pages: 1969 - 1973



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