International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


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Research Paper | Chemistry | India | Volume 3 Issue 7, July 2014 | Popularity: 6.9 / 10


     

Stability Indicating Rp-Hplc Method Development and Validation for Simultaneous Analysis of Quinapril and Hydrochlorthiazide in Bulk and Tablet Dosage Form

Shabeen Taj A, Rubesh Kumar S


Abstract: A simple, rapid, accurate, specific and sensitive RP-HPLC method has been developed and validated for the simultaneous estimation of Quinapril (QUI) and Hydrochlorthiazide (HCTZ) in bulk drug and pharmaceutical dosage form. The chromatographic separation was performed on thermoscientific Inertsil ODS C18 column (150mm4.6mm, 5m particle size) using a mobile phase of phosphate buffer: acetonitirle (26: 74v/v), at a flow rate of 1.2ml/min at ambient temperature with the detection wave length at 210nm. The retention times of QUI and HCTZ were 3.76 min and 2.31 min respectively. The linearity was performed in the concentration range of 25-150 g/ml (QUI) and 31.25-187.5 g/ml (HCTZ) with a correlation coefficient of 0.999 respectively. The percentage purity of Quinapril and Hydrochlorthiazide was found to be 99.8 and 100.4 % w/v respectively. The proposed method has been validated for specificity, linearity, precision, accuracy and robustness were within the acceptance limit according to ICH guidelines and the developed method was successfully employed for routine quality control analysis in the bulk and combined pharmaceutical dosage forms.


Keywords: Quinapril, Hydrochlorthiazide, RP-HPLC, Validation


Edition: Volume 3 Issue 7, July 2014


Pages: 1134 - 1139



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Shabeen Taj A, Rubesh Kumar S, "Stability Indicating Rp-Hplc Method Development and Validation for Simultaneous Analysis of Quinapril and Hydrochlorthiazide in Bulk and Tablet Dosage Form", International Journal of Science and Research (IJSR), Volume 3 Issue 7, July 2014, pp. 1134-1139, https://www.ijsr.net/getabstract.php?paperid=20141063, DOI: https://www.doi.org/10.21275/20141063



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