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Research Paper | Genetics Science | Egypt | Volume 3 Issue 6, June 2014 | Rating: 6.7 / 10
Attenuation of Nano-Tio2 Induced Genotoxicity; Mutagenicity and Apoptosis by Chlorophyllin in Mice Cardiac Cells
Hanan Ramadan Hamad Mohamed
Abstract: Despite uses of titanium dioxide (TiO2) particles in various consumer and medical products therapy increasing human daily exposure; little studies were conducted on its cardiotoxicity. Thus; this study was designed to investigate the possible modulation of nano-TiO2 induced genotoxicity; mutagenicity and apoptosis by chlorophyllin (CHL) coadministration in mice cardiac cells. Mice were injected into the abdominal cavity with TiO2 (500; 1000 and 2000 mg/kg b. w) suspended either in deionized dist. water or in CHL (40 mg/kg b. w) solution for five consecutive days and scarified 24 hour after the last injections. CHL co-administration resulting in significant reductions in tail length; %DNA in tail and tail moment that was highly elevated in nano-TiO2 treated groups in a dose dependent manner; also the appearance of both apoptotic fragmentized Laddered and smeared DNA on agarose gel was returned to the normal un-fragmentized appearance. The observed dose dependent high incidence of mutations inductions in p53 exons (5-8) and myocardial cells infiltrations by inflammatory cells; hemorrhage and congested blood vessels by nano-titanium was attenuated by CHL co-administration. Finally; CHL improved the antioxidant defense system indicated by significant reduction in malondialdiahyde level and increases in reduced glutathione level and glutathione peroxidase activity that were disrupted in nano-titanium treated groups. In conclusion: nano-TiO2 particles induced genotoxicity; mutagenicity and apoptosis were attenuated by CHL co-administration in mice cardiac cells. It is recommended for further studies to detect the CHL dose that completely normalize nano-titanium induced cardiotoxicity.
Keywords: TiO2 nanoparticles, genotoxicity, mutagenicity, p53, apoptosis, mice cardiac cell
Edition: Volume 3 Issue 6, June 2014,
Pages: 2625 - 2636