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Research Paper | Chemistry | Sudan | Volume 8 Issue 5, May 2019 | Popularity: 6.9 / 10

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Development and Validation Method for the Determination of Sildenafil Citrate Tablets Drugs by Using High Performance Liquid Chromatography (HPLC) in Pharmaceutical Formulation

Alrasheed. AW. Mohammed, Salah Elnaiem Mohammed, Hanaa. S. Boshra, Muzamil Hassaballa Abdelmula, Munzir Omer Mohammed, Ahmed Mohammed Ali Hammad

Abstract: The objective of this is to describe the optimization, validation, and application of spectrochromatographic techniques for determination of Sildenafil Citrate in their pharmaceutical formulation (tablets). A simple, rapid, accurate and sensitive c spectrochromatographic has been developed and validated. The method is a direct spectrochromatographic method based on the chromatographic separation of Sildenafil citrate compound. This chromatographic method was developed by using inertstil C18 (250 *4.6 mm), 5m column with a mobile phase consisting of 0.2 M ammonium acetate and Acetonitrile in (1: 1) ratio. The flow rate was adjusted at 1.0 ml/min, injection volume 20 L, with UV-Detector the maximum absorption peak (max) at 240 nm, temperature, at ambient temperature, and retention time was found to be 6.002 min. Under the optimized condition, beers law correlating the absorbance (Y) with concentration (X) was obeyed in the range of 0.014 to 0.035 g/ml.

Keywords: Sildenafil Citrate Tablets

Edition: Volume 8 Issue 5, May 2019

Pages: 2137 - 2146

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