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M.Tech / M.E / PhD Thesis | Medicine Science | Indonesia | Volume 5 Issue 10, October 2016 | Popularity: 6.7 / 10

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Study of Hepatitis B Virus Desoxyribonucleic Acid in Blood Donors with Negative Hepatitis B Surface Antigen and Positive Anti Hepatitis B Surface

Dwi Yulia, Ellyza Nasrul, Nasrul Zubir, Yanwirasti

Abstract: STUDY OF HEPATITIS B VIRUS DESOXYRIBONUCLEIC ACID IN BLOOD DONORS WITH NEGATIVE HEPATITIS B SURFACE ANTIGEN AND POSITIVE ANTI HEPATITIS B SURFACE DWI YULIA, ELLYZA NASRUL, NASRUL ZUBIR, YANWIRASTI MEDICAL FACULTY, ANDALAS UNIVERSITY, PADANG, INDONESIA Infection of HBV is still one of the highest burden disease in the world. It is estimated that two billions of the worlds populations has had contact HBV. HV related complications such as cirrhosis and hepatocellular carcinoma (HCC). One of the contamination of HBV was transfution of blood that infected by HBV. The aim of study to determinmine there is HBV DNA at blood donors with negative Hepatitis B surface antigen negatif and positive anti hepatitis B surface. Desain of this study is deskriptive cross sectional. The study was conducted at clinical laboratory, UTD RSUD Prof DR MA Hanafiah SM Batusangkar and Sawahlunto and Biomedical Laboratory, Faculty of Medicine, Andalas University. From Oktober 2014 to March 2016. Specimen of this study are blood donors with negative Hepatitis B surface antigen and positive anti hepatitis B surface that tested using strip by kromatografi method. HBV DNA was tested by konventional PCR and occult B Hepatitis Infection by RT PCR. The sekuensing of nukleotida was sent to Macrogene, Korea. The result of this study is there is no HBV DNA at blood donors and there is no occult B Hepatitis B at blood donors with negative HBsAg and positive anti HBs. This test using kontrol positif that estimated by sekuensing nukleotide of HBV. The conclusion of this study is there is no HBV DNA and occult B Hepatitis at blood donors with negative HBsAg and positive anti HBs. To suggest for using anti HBs test at blood donors with negative HBsAg to stoped transmision of HBV. Infection of Hepatitis B Virus is still one of the highest burden disease in the world. It is estimated that two billions of the worlds populations has had contact Hepatitis B Virus. Hepatitis B Virus related complications such as cirrhosis and carcinoma. One of the contamination of Hepatitis B Virus was blood donors that infected by Hepatitis B Virus. The aim of study to determine there is Hepatitis B Virus DNA at blood donors with negative Hepatitis B surface antigen negative and positive anti hepatitis B surface. Design of this study is cross sectional. The study was conducted at clinical laboratory, Unit blood donors in hospital and Biomedical Laboratory, Faculty of Medicine, West Sumatra, From October 2014 to March 2016. Specimen of this study are blood donors with negative Hepatitis B surface antigen and positive anti hepatitis B surface that tested using test strip. method. Hepatitis B Virus DNA was tested by conventional Polymerase Chain Reaction and occult B Hepatitis Infection by RT Polymerase Chain Reaction. The result of this study is there is no Hepatitis B Virus DNA at blood donors and there is no occult B Hepatitis B at blood donors with negative Hepatitis B surface Antigen and positive anti Hepatitis B surface. The conclusion of this study is there is no Hepatitis B Virus DNA and occult B Hepatitis at blood donors with negative Hepatitis B surface Antigen and positive anti Hepatitis B surface.

Keywords: HBV DNA, HBsAg, Anti HBs, PCR, RT-PCR, Blood donors

Edition: Volume 5 Issue 10, October 2016

Pages: 1450 - 1463

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