International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


Downloads: 136 | Views: 287

Research Paper | Agronomy | Canada | Volume 5 Issue 8, August 2016 | Popularity: 6.3 / 10


     

Phenotypic, Biochemical and Molecular Diversity of Phoma lingam Fungal Isolates Collected from Saskatchewan, Canada

Rafiq Ahad, Ginette Sguin-Swartz, Richard K. Gugel, Birger Koopmann


Abstract: The population of Phoma lingam associated with blackleg disease of canola (Brassica napus) is highly polymorphic, and infect various Brassicaceae species, including Thlaspi arvense (stinkweed). In order to determine the population composition and pathogenicity of P. lingam infecting T. arvense, 18 isolates recovered from T. arvense (designated Thl isolates) from different crop fields of Saskatchewan were compared with an aggressive (IBCN 2) and eleven less/non-aggressive reference strains. No common morphological, biochemical and pathogenic characteristics were found between Thl isolates and IBCN 2 strain. DNA fingerprinting and phylogenetic analysis of ITS-rRNA gene sequences revealed that the Thl P. lingam population of Saskatchewan is highly heterogeneous and comprised of at least eight genotypes. Over 50 percentage of Thl isolates clustered with different less/non aggressive reference strains belonging to L. biglobosa. Interestingly, a group of Thl isolates clustered with an aggressive reference strain, IBCN 2. Therefore, regular monitoring is required to determine if the Thl P. lingam population of western Canadian provinces is migrating from non-aggressive toward aggressive state.


Keywords: Blackleg disease, genetic diversity, ITS-rRNA sequence, oilseed rape, Phoma lingam


Edition: Volume 5 Issue 8, August 2016


Pages: 436 - 443


DOI: https://www.doi.org/10.21275/ART2016847



Make Sure to Disable the Pop-Up Blocker of Web Browser




Text copied to Clipboard!
Rafiq Ahad, Ginette Sguin-Swartz, Richard K. Gugel, Birger Koopmann, "Phenotypic, Biochemical and Molecular Diversity of Phoma lingam Fungal Isolates Collected from Saskatchewan, Canada", International Journal of Science and Research (IJSR), Volume 5 Issue 8, August 2016, pp. 436-443, https://www.ijsr.net/getabstract.php?paperid=ART2016847, DOI: https://www.doi.org/10.21275/ART2016847



Similar Articles

Downloads: 115

Research Paper, Agronomy, India, Volume 4 Issue 7, July 2015

Pages: 2585 - 2587

Genetic Divergence among Advanced Breeding Lines of Medium Duration Rice (Oryza Sativa L.)

U. Praveen Kumar, Suresh B.G., Ankit Kumar.M, Swamy.P.Y.

Share this Article

Downloads: 120

Research Paper, Agronomy, Bulgaria, Volume 5 Issue 5, May 2016

Pages: 1481 - 1486

Chemical and Microbiological Studies for Determination the Influence of Fertilizers Produced by ?Agropolychim? Ad on Winter Common Wheat and Oilseed Rape

Dragomir Plamenov, Pavlina Naskova, Bojka Malcheva, Yordan Iliev

Share this Article

Downloads: 134

Research Paper, Agronomy, Tunisia, Volume 5 Issue 1, January 2016

Pages: 126 - 128

Optimization of Reproducible Biochemical Genotype for Population: The Case of Trifolium Alexandrinum L., Fabacae

S. Rouz, F. Ben Jeddi, M.Zouaghi, Z. Ghrabi-Gammar

Share this Article
Top