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Research Paper | Nursing | Indonesia | Volume 6 Issue 5, May 2017 | Popularity: 6.8 / 10
Influenced of Epiphyte Flavonoid Tea Scurrula Artropurpurea Danser in Apoptosis Induction for Tissue Isolation of Cervical Cell Cancer
Budi Susatia
Abstract: Cervical carcinoma is a type of cancer to two experienced by women and the leading cause of death for women in the world, extracts of loranthus tea (Scurrula artropurpurea) have flavonoids, compounds to which this activity in flavonoids induces apoptosis and suppresses the proliferation in cervical carcinoma cell culture. This research aims to identify the potential of the flavonoid compounds in tea induce apoptosis loranthus and inhibit proliferation in cervical carcinoma cell culture. This type of research is used in experiments which is conducted in the laboratory of Faculty of Pharmacy University of Airlangga. The sample used is cervical carcinoma HeLa cells (the cells He La). These cells are epithelial cells morphology that already infect Human Papilloma Virus (HPV) type 18. Then do a test with a Sitotoksisitas Assay and MTT Assay Apoptosis by Double Staining Method. The results of the research on proliferation and apoptosis in cells was reported in descriptive as follows 1) Extract tea Scurrula loranthus artropurpurea (Blume) Danser good fraction of ethanol, chloroform, or n-heksan was able to inhibit proliferation with the strongest potential shown by extract chloroform with IC50 values of 96.160 g/ml of ethanol, followed by the value of the IC50 of g/ml 298.801 and n-heksan with IC50 values of 489.661 g/ml.2) Extracts tea Scurrula loranthus artropurpurea (Blume) Danser good fraction of ethanol, chloroform, or n-heksan is able to induce apoptosis in the cell He-La has the potential for chloroform Fraction. Induces apoptosis levels where most powerful 22.413 g/ml (23.31 % of IC50) able to cause apoptosis of 75.72 %, followed by the tea ethanol fraction loranthus and n heksan. Further research is needed to find out the mechanism of flavonoid in inhibiting the proliferation and inducing apoptosis in cervical cancer cells.
Keywords: Loranthus extract tea Scurrula artropurpurea, Flavonoids, Apoptosis, Proliferation, Cell cervical carcinoma
Edition: Volume 6 Issue 5, May 2017
Pages: 913 - 918
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