International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


Downloads: 126 | Views: 244

Research Paper | Biochemistry Science | Egypt | Volume 6 Issue 6, June 2017 | Popularity: 6.2 / 10


     

Generation of Cholinergic Neurons from Human Olfactory Bulb Neural Stem Cells Using BMP9 Treatment

Camelia A. Abdel Malak, Hany E. Marei, Mohammad Omran, Asmaa A. Moeit


Abstract: The aim of the present study was to obtain a predominantly pure population of cholinergic neurons from human olfactory bulb neural stem cells (hOBNSCs) using diffusible ligands and to study the gene expression profile for the key genes involved in this transformation (CHAT, CHAT1, NF-L, IIITUB, MAP2, NGFR). Differentiation of hOBNSCs, which was initiated by of BMP9, significantly increased expression of these genes, with highest expression in NGFR followed by CHAT, CHAT1, MAP2, NF-L, and lowest expression in IIITUB. These data provide, for the first time, the ability of hOBNSCs to differentiate in vitro into cholinergic neurons expressing CHAT, MAP2, NF-L, IIITUB, CHAT1, NGFR genes and this can help us to know the potential mechanism of cholinergic differentiation of hOBNSCs which will be helpful when these cells used in treatment of neurodegenerative diseases.


Keywords: human olfactory bulb, neural stem cells, cholinergic neurons, gene expression


Edition: Volume 6 Issue 6, June 2017


Pages: 751 - 754



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Camelia A. Abdel Malak, Hany E. Marei, Mohammad Omran, Asmaa A. Moeit, "Generation of Cholinergic Neurons from Human Olfactory Bulb Neural Stem Cells Using BMP9 Treatment", International Journal of Science and Research (IJSR), Volume 6 Issue 6, June 2017, pp. 751-754, https://www.ijsr.net/getabstract.php?paperid=ART20174170, DOI: https://www.doi.org/10.21275/ART20174170