International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


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Research Paper | Medicine Science | Indonesia | Volume 6 Issue 6, June 2017 | Popularity: 6.9 / 10


     

Effect of Electric Fields on Normal Fibroblast (BJ) Cells

Omat Rachmat, Ilma Fidayanti, Dewi Ratih, Endang Sutedja, Darmadji Ismono, Nucki N Hidajat


Abstract: Background Electric fields (EFs) has been reported to affect living tissues. Effects of EFs as anticancer have been recently developed. Designing a therapy that specifically recognize and kills cancer cells without affecting normal cells, has been a recent objective in theranostics. Thus, this study aimed to evaluate the effect of EFs on normal fibroblast cells (BJ cells). Method Cells were incubated for 2, 4, and 6 days with frequency of 50 KHz, 100 KHz, 150 KHz, 200 KHz, 300 KHz. Parameters measured were cells number and viability. Cells number and viability assesment was performed with MTS. Results Number of BJ cells increased each day, compared to 0 day. Number of cells in each day showed no significant difference among treatment of EFs. There was no significant difference of cells viability among treatments each day, and among treatments-0 days, that indicate EFs showed no toxicity toward BJ cells. Conclusion The result of present study showed there was no significant difference among treatment of EFs in normal fibroblast cells. These results indicate EFs did not affect proliferation in normal cell.


Keywords: Electric fields, fibroblast


Edition: Volume 6 Issue 6, June 2017


Pages: 626 - 629



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Omat Rachmat, Ilma Fidayanti, Dewi Ratih, Endang Sutedja, Darmadji Ismono, Nucki N Hidajat, "Effect of Electric Fields on Normal Fibroblast (BJ) Cells", International Journal of Science and Research (IJSR), Volume 6 Issue 6, June 2017, pp. 626-629, https://www.ijsr.net/getabstract.php?paperid=ART20174172, DOI: https://www.doi.org/10.21275/ART20174172



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