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M.Tech / M.E / PhD Thesis | Microbiology | Iraq | Volume 6 Issue 7, July 2017 | Popularity: 6.7 / 10

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Bacteriological and Immunological Study of Rheumatoid Arthritis

Dr. Suaad Khalil Ibrahim, Teeba Fares Hadi

Abstract: The current study included the collection of 175 samples of blood (Urea - blood) of patients with rheumatoid arthritis from Al Al-Kindy Teaching Hospital, Baghdad Teaching Hospital and Al-Imamian Al - Kadhimyain Medical City in Baghdad from both sexes with different ages at the period between 1/10/2016 -1/2/2017. Bacterial growth results showed that 80 % of urea for bacterial transplantation were positive results, while the number of samples showing no bacterial growth was 20 %. The bacterial isolation evaluate for morphological testes and biochemical microscopy, as well as identification by Api system. The highest frequency of inflectional bacteria was E. coli (41.97 %), followed by E. cloacae (21.25 %), P. aeruginosa (12.5 %), Salmonella (10 %), K. pneumoniae (7.5 %) and S. marcescens (6.25 %). The concentration of some cytokines IL-6, TNF was measured by using ELISA technical. The results showed significant decrease at P0.05 in level of IL-6 for patient serum (23.76 14.01 pg/ml) compared with healthy serum (209.11 76.99) pg /ml. The result of TNF showed an increase significantly at P0.05 in level of serum for rheumatoid arthritis patient (437.68 16.80 pg /ml) compared with healthy serum (370.31 26.87 pg /ml). The study found that reverse relation between IL-6 and TNF.

Keywords: Rheumatoid arthritis, IL-6, TNF-

Edition: Volume 6 Issue 7, July 2017

Pages: 1483 - 1487

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