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Analysis Study Research Paper | Botany | India | Volume 12 Issue 5, May 2023 | Popularity: 5 / 10

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Factors Affecting Micropropagation of Maytenus Emarginata: A Plant of Stressed Ecosystem

Dr. Narpat Singh Deora

Abstract: Types of media (MS basal, MS three- fourth, MS half, B5 and WP), explants and growth regulators (auxin, cytokinin) with various concentration played important role in the bud breaking or multiple shoot induction under in vitro. Out of various media and growth regulators tested the maximum number of explants were responded on MS full strength medium supplemented with NAA 0.1 mg/l + BAP 5.0 mg/l +additives. Different type of cytokinin (2-IP, KN, BAP) with concentration from 0.25-2.5 mg/l) were tested but best result from subcultured shoot were found on MS medium supplemented with IAA 0.1 mg/l + BAP 2.5 mg/l+ additives (ascorbic acid 50 mg/l, citric acid 25 mg/l L-arginine 25 mg/l and adenine sulphate 25 mg/l). On this medium the nodal shoot segment explants which were taken from lopped and managed tree were found the best for shoot induction. These cultures were incubated at 28?2 C under regime of 12 h photoperiod of light for shoot induction.

Keywords: in vitro, medium, subculture, inoculation, explant

Edition: Volume 12 Issue 5, May 2023

Pages: 1131 - 1132

DOI: https://www.doi.org/10.21275/MR23513112038

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