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Research Paper | Zoology | India | Volume 14 Issue 1, January 2025 | Popularity: 8.5 / 10
Isolation, Identification and Characterization of Keratinolytic Bacteria from Poultry Waste
Kratika Sharma
Abstract: The poultry sector is an important sector for all the economies of the world but this sector also adds to a bulk of poultry wastes. The study will enhance the understanding of decomposition process of poultry waste and mitigation strategy. This research will provide a platform for the scholars to concentrate on the keratolytic property of the isolated microbes which helps in the decomposition of feathers, litter, and other poultry wastes. Further chemical and morphological characteristics of these isolated microbes are analysed to understand the biological process of breaking the keratinous enzymes contained in the poultry wastes and their scientific utilization for producing other useful products such as cattle fodder or renewable energy generation etc. Soil samples were collected from Poultry farm of Dausa and Jaipur district in Rajasthan. Isolation and identification of bacterial pathogens were carried out based on their growth pattern and colony characteristics on selective and differential media. Gramstaining, Catalase, Oxidase, Urease and motility tests were carried out to confirm the findings. We identified four separate types of keratinolytic bacteria in this study. The isolates were obtained, they were cultivated on feather broth to determine keratinase activity. Keratinase being found in microbial culture of keratin - rich wastes, which were found to be applicability for enzyme production & waste treatment and providing value to these agro - industrial leftovers.
Keywords: Keratin, Degradation, Feather Meal Broth, Feather Meal Agar
Edition: Volume 14 Issue 1, January 2025
Pages: 1133 - 1140
DOI: https://www.doi.org/10.21275/MR25125221856
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