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Research Paper | Biomedical Sciences | Indonesia | Volume 12 Issue 2, February 2023 | Rating: 5.1 / 10
Profile of Adenocarcinoma in Cohort of Female Lung Cancer Related Second Hand Smoker
R. A Henny Anggriani | Noni Novisari Soeroso [3] | Setia Putra Tarigan [2] | Putri C Eyanoer [2] | Hidayat [35]
Abstract: Background: Environmental tobacco smoker is the major significant factor in developing lung cancer in females never smokers. There are two products of cigarette smokes; mainstream and side stream smoke that both of these smoke will be metabolyzed by CYP2A6 in both active and passive smoker. Further, adenocarcinoma is the most common histology in lung cancer related to female never smoker and strongly related to nicotine metabolism in the carcinogenesis. Objectives: This study aimed to assess the genetic polymorphism of CYP2A6 and cytology characteristics in female second - hand smokers who suffered from lung cancer among the North Sumatera population. Methods: A cross sectional study was carried out in three centers of cancer hospital in Medan with a total of 53 subjects enrolled in this study from purposive sampling. The diagnosis of lung cancer was confirmed by clinical, radiology, cytology, and histopathology preparation. The CYP2A6 gene was examine by PCR - RFLP from blood. Data were analyzed with the Conditional Logistic Regression test using Epi info 7.0 software. Results: A total 53 subjects diagnosed with lung cancer, with 51 participants had adenocarcinoma type. The most common genotype is wild type, including *1A/*1A (31.3%), *1A/*1B (27.4%), *1B/*1B (39.2%). Meanwhile, only 1 subject had mutant type *4A/*4A (1.9%). For allele, only 1 subject had mutant type *4A (1.9%), while the wild type were counted as *1A (58.8%), *1B (39.2%). There was no significant associations between genotype of CYP2A6 and the incidence of lung adenocarcinoma (p = 0.61). Neither was the alelle of CYP2A6 and the incidence of lung adenocarcinoma (p = 0.25). Conclusion: Adenocarcinoma is the most common histology in female never smoker. Yet, there was just 1 subject had the mutant type of CYP2A6, so this study cannot associate between allele and genotype of lung cancer and the incidence of lung adenocarcinoma.
Keywords: Polymorphism, CYP2A6, Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR - RFLP), Female Second Hand Smokers, Lung Cancer, Cytology of Lung Cancer
Edition: Volume 12 Issue 2, February 2023,
Pages: 567 - 569