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Research Paper | Biotechnology | India | Volume 12 Issue 6, June 2023 | Rating: 5.3 / 10
Estimation of Salivary Levels of Interleukin-35 and Osteoprotegerin in Periodontitis - A Cross Sectional Study
Maniyammai. A. S. | Dr. R. Renuka Devi | Dr. H. Esther Nalini | Dr. G. Kokila | Dr. S. Tamil Selvi | Dr. C. Nitya Kala
Abstract: Background: Periodontitis is a multifactorial disease primarily caused by microbial biofilm containing different colonies of microorganisms. Inflammation and bone loss are considered the hallmarks of periodontal disease (PD). The evolution of biomarkers for early detection of periodontal disease and to quantitate disease progression would be highly desirable. It helps to reveal a hidden lethal threat before the disease becomes complicated. Aim & Objective: The aim of the present study is to estimate and correlate the salivary levels of IL-35 and Osteoprotegerin with the periodontal disease severity. Materials and methods: A total of 44 individuals with the age of 25-60 years were included in the study. Group 1 (n=22) were periodontally and systemically healthy individuals and Group 2 (n=22) systemically healthy individuals with periodontitis. Clinical parameters evaluated were Plaque index (PI), Gingival bleeding index (GBI), Probing pocket depth (PPD), Clinical attachment levels (CAL). Un-stimulated salivary samples were collected and analyzed for IL-35 and Osteoprotegerin using sandwich ELISA method. Statistical analysis were performed. Results: The levels of IL-35 and OPG were detected in both the groups. Statistically significant differences were found between IL-35 and OPG salivary levels, and clinical parameters such as Plaque index (PI), Gingival bleeding index (GBI), Probing pocket depth (PPD), Clinical attachment levels (CAL). The salivary levels of IL-35 (13.519?2.020) and OPG (3.327?0.437) was significantly higher in group 2 patients and it was statistically significant with p value of 0.003 and 0.004 respectively. Conclusion: The results of our findings suggests that increase in the salivary levels of IL-35 and OPG shows a predictable association with the presence of inflammation. However, longitudinal studies should be carried out with larger population to validate the role of IL-35 and OPG in periodontitis which may be useful for screening and diagnosis.
Keywords: Periodontitis, Osteoprotegerin, Interleukin-35, Inflammation, Cytokines
Edition: Volume 12 Issue 6, June 2023,
Pages: 829 - 833