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Research Paper | Dentistry | United Kingdom | Volume 12 Issue 5, May 2023 | Popularity: 4.4 / 10
Biocompatibility of Silicate Based Root Canal Sealers on Human Fibroblast Cells - In Vitro Study Based on Direct Visual Observation
Dr Aswiyaa Muhamed Ilyas
Abstract: Tricalcium silicate sealers which have been launched on the market claim to have better biocompatibility compared to other sealers which are regularly used. In vitro study was conducted to compare the biocompatibility and cytotoxicity of the recently launched calcium silicate-based root canal sealers (BioRoot RCS and Totalfill BC) with AH+ which is commonly used in the clinical practice and is the ?gold standard? using direct visual observations. Discs prepared from the respective sealers were placed with MM1 cell line (human gingival fibroblast) and WPS9 cell line (human periradicular fibroblast) for seven days to check the biocompatibility of these materials. Visual observations shows that AH+ sealer is the most toxic as there is an increased amount of cell death with respect to both the cell lines and the tricalcium sealers caused more morphological changes in MM1 when compared to WPS9.
Keywords: Biocompatibility, Tricalcium silicate sealers, fibroblasts, AH+, visual observation
Edition: Volume 12 Issue 5, May 2023
Pages: 2087 - 2098
DOI: https://www.doi.org/10.21275/SR23521050351
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