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Research Paper | Gynaecology | Iraq | Volume 12 Issue 7, July 2023 | Rating: 4.9 / 10
Phylogenetic Analysis of SARS-CoV-2 Isolated from Pregnant Iraqi Women
Dr. Nagham Taleb Maki Al-Ibraheemi | Dr. Mohammed Abdulwahab Ati Al-Askeri
Abstract: The aims of this study were to explore as well as follow down the SARS-CoV-2 patients in Iraq using modern molecular and phylogenetic. We have studied thirty five definite cases of COVID-19 pregnant patients for the phylogenetic evaluation of the SARSCoV-2 in Iraq. The main genes of spike (s) and ?RNA-dependent RNA polymerase (RdRp) genes? were got amplified via one-step RT-PCR then sequenced based on Sanger sequencing method. Bioedit and Mega bioinformatics software were applied for both sequences alignments and phylogenetic relationship building up frame. The results indicated a possible similarity flanked by Iraqi and Chinese incoming strains.
Keywords: COVID-19, RT-PCR, Molecular diagnosis, mutations, Iraq
Edition: Volume 12 Issue 7, July 2023,
Pages: 981 - 984