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Research Paper | Pharmaceutical Science | India | Volume 13 Issue 2, February 2024 | Rating: 5.7 / 10
Chaulmoogra Oil Nanoemulsion and in Vitro Anti-Cancer Studies Using MCF 7 Cell Line
Seema Gadge | Priyanka Varade | Rini Punathil | Karthika Gouthaman | Jincy Joy
Abstract: Nanotechnology comprises technological developments on the nanometer range of usually 0.1 -100nm. The pharmaceutical developed on the basis of nanotechnology is referred to as ?NANOPHARMACEUTICALS?. Various nanopharmaceuticals currently being used or in the process of development are Nanoemulsions, Nanosuspensions, Nanospheres, Nanotubes, Nanoshells, Nanocapsules, lipid nanoparticles and dendrimers. Chaulmoogra oil Nanoemulsion was prepared by High pressure homogenisation technique. In this method, homogenisation involves the forcing of the emulsion under pressure through a valve having a narrow aperture. Nanoemulsions are thermodynamically and physically stable systems and are formed at a particular concentration of oil, surfactant and water, making them stable to phase separation, creaming or cracking. Thus, the formulations were tested for their physical stability using centrifugation, heating-cooling cycle and freeze-thaw cycle. The particle size of Chaulmoogra oil Nanoemulsion was found to be 200 ? 800(d.nm). The Zeta potential values of the formulated Nanoemulsion were obtained as -0.0666mV and -0.0327mV. The viscosity of Nanoemulsions was found to be low as the consistency should be low for better application of the Nanoemulsion in topical administration. The formulated Chaulmoogra oil Nanoemulsion were screened for antimicrobial activity against both gram positive and gram negative organisms by cup-plate method. The Chaulmoogra oil Nanoemulsion exhibited a significant antioxidant activity against DPPH method at 10% V/V. The antioxidant activity of the samples showed a direct dose- response relationship. In-vitro cytotoxic activity against human breast cancer cell line MCF-7 was evaluated at different concentration (0.1% V/V, 1%V/V and 10% V/V) by MTT assay. The in-vitro screening of the formulated nanoemulsions showed potent cytotoxic activity against the human breast cancer cell line. The cytotoxicity analysis of the samples showed a direct dose-response relationship; cytotoxicity increased at higher concentration.
Keywords: Chaulmoogra oil, Nanoemulsion, Viscosity, High pressure homogenizer, Brookfield viscometer
Edition: Volume 13 Issue 2, February 2024,
Pages: 423 - 430