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Research Paper | Microbiology | India | Volume 13 Issue 5, May 2024 | Rating: 5 / 10
Detection of Rifampicin Resistance in Pulmonary Tuberculosis by Molecular Methods in a Tertiary Care Hospital
Dr. I. Divya Sri | Dr. N. Padma Priya [3] | Dr. Sireesha Chava [2] | Dr. Sulakshana Sony Cheemala
Abstract: Introduction: Tuberculosis is the 2nd major cause of death and a significant public health concern among infectious diseases. India contributes to over 25% of the world's tuberculosis cases. Effective disease control depends on the early and rapid diagnosis of tuberculosis (TB) and the identification of antibiotic resistance. The CBNAAT and the LPA (Line Probe Assay) have been approved by the WHO for the rapid diagnosis of DRTB. Materials and Methods: A prospective study done in the department of Microbiology, GMC, Ongole. Sputum samples were collected from 1600 Presumptive TB and 350 Presumptive DR - TB patients attending at GGH, Ongole. CBNAAT was done on all sputum samples to detect tuberculosis and its RIF resistance. FL - LPA was done for CBNAAT - positive samples to detect RIF resistance mutation in the rpoB gene along with INH resistance mutations in the katG and inhA genes. SL - LPA was done for FL - LPA positive samples to detect FQ resistance mutations in gyrA and gyrB genes along with SLI drugs resistance mutations in rrs and eis genes. Results: Among 1600 presumptive TB samples, 197 (12.3%) positives for MTB, all were sensitive to Rifampicin by CBNAAT and rpoB gene mutation not detected by FL - LPA. Among 350 presumptive DRTB samples, 76 (21.7%) positives for MTB, 8 (10.5%) showing Rifampicin resistance by CBNAAT. Out of 76 CBNAAT MTB detected samples FL - LPA detected rpoB gene mutation in 10 samples (8 same as detected in CBNAAT and also in other 2 samples). Out of 10 RR samples, SL - LPA detected gyrB gene mutation showing FQ resistance in one sample. Conclusion: The current study suggests that while CBNAAT is thought to be the best approach for identifying MTB and detecting rifampicin resistance, it is important to keep in mind that resistance to isoniazid monotherapy and second - line drugs is also very prevalent. In these situations, LPA is more important.
Keywords: MTB, CBNAAT, LPA, RIF resistance
Edition: Volume 13 Issue 5, May 2024,
Pages: 69 - 72