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Case Studies | Pathology | India | Volume 13 Issue 5, May 2024 | Rating: 5.1 / 10

Assessing the Effectiveness of Xylene-Free Deparaffinization in Hematoxylin and Eosin Staining

Sreeganesh A S ^[3] | Ramjeela S ^[5]

Abstract: Xylene is the deparaffinizing agent used in Hematoxylin and Eosin staining. It is expensive, dangerous, and will contaminate the workplace. There's also the disposal issue. Therefore, using a low-cost and environment friendly deparaffinization replacement is highly beneficial. To assess how well xylene-free sections perform in terms of yielding sufficient H and E staining in a straightforward, economical, and faster manner in comparison to traditional H and E sections, forty paraffin blocks were taken into account, a piece was stained using the traditional H and E procedure and another using the H and E method without xylene. Slides were graded according to the following criteria: uniformity, clarity, crispness, nuclear and cytoplasmic staining.

Keywords: Hematoxylin, Eosin, Xylene, Deparaffinization

Edition: Volume 13 Issue 5, May 2024,

Pages: 1143 - 1145