Downloads: 7 | Views: 98 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Case Studies | Pathology | India | Volume 13 Issue 5, May 2024 | Popularity: 5.2 / 10

Rate This Article!      

Assessing the Effectiveness of Xylene-Free Deparaffinization in Hematoxylin and Eosin Staining

Sreeganesh A S, Ramjeela S

Abstract: Xylene is the deparaffinizing agent used in Hematoxylin and Eosin staining. It is expensive, dangerous, and will contaminate the workplace. There's also the disposal issue. Therefore, using a low-cost and environment friendly deparaffinization replacement is highly beneficial. To assess how well xylene-free sections perform in terms of yielding sufficient H and E staining in a straightforward, economical, and faster manner in comparison to traditional H and E sections, forty paraffin blocks were taken into account, a piece was stained using the traditional H and E procedure and another using the H and E method without xylene. Slides were graded according to the following criteria: uniformity, clarity, crispness, nuclear and cytoplasmic staining.

Keywords: Hematoxylin, Eosin, Xylene, Deparaffinization

Edition: Volume 13 Issue 5, May 2024

Pages: 1143 - 1145

DOI: https://www.doi.org/10.21275/SR24517233222

Make Sure to Disable the Pop-Up Blocker of Web Browser

Click to Cite this Article

Similar Articles