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Review Papers | Pathology | India | Volume 13 Issue 7, July 2024 | Rating: 4.1 / 10 | Rate this Article
Pentachrome Staining: A Comprehensive Review of the Novel Method for Collagen and Sulfated Mucopolysaccharides
Abstract: One of the most prevalent fibers in the extracellular matrix is collagen, which is also home to sulfated mucopolysaccharides. Sulfated mucopolysaccharides are also found in certain secretory glands and goblet cells. This article's goal is to provide a review of novel staining technique that can identify these two macromolecules in the same sample at the same time. Using the given procedure, tissues are stained in five primary colors: red for collagen, violet for sulfated mucopolysaccharides, yellow for red blood cells, orange for muscle, and green for nuclei. In this study, xylene deparaffinized tissue sections are used for the pentachrome staining. Xylene is expensive, dangerous, and will contaminate the work place. There?s also the disposal issue. In some other studies, eco-friendly substitutes are successfully used for the deparaffinization of sections for H&E staining. Therefore, for the pentachrome staining also xylene free deparaffinization may be used. Recommendations for future research are made in order to overcome this knowledge gap.
Keywords: collagen, sulfated mucopolysaccharides, pentachrome
Edition: Volume 13 Issue 7, July 2024,
Pages: 114 - 115