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Research Paper | Botany | India | Volume 13 Issue 8, August 2024 | Rating: 5.6 / 10
The Histochemical Study of Microsporogenesis in Solanum seaforthianum using PAS Method
Deepa R. Mesta
Abstract: Present study is on the microsporogenesis of Brazilian nightshade Solanum seaforthianum with histochemical analysis. It involves differentiation of anther wall and sporogenous cells. The anther wall development process includes differentiation of wall layers such as the epidermis, endothecium, middle layers, and tapetum. All the change that takes place in the microsporangium during anthesis can be distinguished into three stages 1. Early differentiation, 2. Differentiation during meiosis 3. Post meiotic differentiation. Histochemical staining is widely used to localize proteins, enzymes, polysaccharides, elements and oxidative stress markers. In Solanum Seaforthianum anther is bilobed, tetrasporangiate. The anther wall follows Basic type of development and tapetum is glandular. Microspore mother cells meiotically divided to produce isobilateral and tetrahedral tetrads are found which is rare in dicots. Anther dehiscence is typically porous. Microsporogenesis is studied along with the metabolic potentials of the cells using histochemical methods. Localization of insoluble polysaccharides in sporogenous and vegetative tissues at different stages of anther development achieved by using PAS staining method.
Keywords: isobilateral, tricolporate, horseshoe shape, papillate
Edition: Volume 13 Issue 8, August 2024
Pages: 1102 - 1105