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Analysis Study Research Paper | Clinical Laboratory Sciences | Indonesia | Volume 13 Issue 9, September 2024 | Popularity: 5.5 / 10

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Cell Morphology Differences in EDTA Tube Blood Between Immediate Examination and 4 Hours Delayed Examination at 20 - 25?

Endrawati K. J., Sastraningsih N. K.

Abstract: Laboratory examination consists of three main processes including pre - analytic, analytical and post - analytic. These three processes are mutually sustainable and need to be considered because they greatly influence the results that are issued. The pre - analytical stage refers to all the steps that must be carried out before the sample can be analyzed. Delay in processing samples that are too long will cause physical and chemical changes which can be a source of errors in the examination. This study aims to analyze the differences in cell morphology in EDTA blood which was examined immediately and delayed for 4 hours at a temperature of 20 - 25?. The type of research used is an experiment. The population is volunteer patients who carry out a complete blood count using EDTA blood who meet the inclusion criteria set by the researcher. Samples were taken using non - probability sampling technique purposive sampling method and analyzed using the Paired Sample T - Test. The results showed that there were differences in cell morphology that were treated immediately and delayed 4 hours, with p - values for erythrocytes, leukocytes and platelets respectively, namely 0.000, 0.002 and 0.000. This value indicates that the p - value <0.05 so that the hypothesis is accepted. Erythrocyte morphological changes that occur are the discovery of burr cells. In leukocytes, the cytoplasm fades, the nucleus breaks and there is cytoplasmic vacuolization. The changes that occur are platelets enlarge to become macro platelets and giant thrombocytes or often called giant platelets. Processing time affects changes in cell morphology, namely the longer the delay time, the greater the number of samples that experience morphological changes compared to samples that are processed immediately.

Keywords: erythrocytes, leukocytes, platelets

Edition: Volume 13 Issue 9, September 2024

Pages: 744 - 748

DOI: https://www.doi.org/10.21275/SR24911124553

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