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Analysis Study Research Paper | Biological Sciences | India | Volume 14 Issue 1, January 2025 | Popularity: 5.6 / 10
Investigating the Genetic Impact of Pioglitazone on Insulin Sensitivity in Human Adipocyte Stem Cells
Dr. Sunaina Gautam, Dr. M. K Ahmad, Dr. Monisha Banerjee
Abstract: Background: The study focuses on Pioglitazone hydrochloride (PH), a PPAR? agonist known for its impact on metabolic diseases. It activates PPAR?, enhancing insulin response in target cells. However, the precise mechanism by which it improves insulin sensitivity remains unclear. Objectives: This research aimed to decipher PH's antidiabetic effects by analyzing key genes (PPAR?, Glut 4, PI3K, TNF?, FASN, IL6) in human adipocytes, vital in diabetes and related issues like atherosclerosis. Methods: Human adipose-derived stem cells were cultured and treated with varying PH concentrations. Cytotoxicity and triglyceride levels were assessed using MTT assays and Oil red O staining. Gene expression (PPAR?, Glut 4, PI3K, TNF?, FASN, IL6) was evaluated through semiquantitative PCR and real-time PCR. Result: At 50 ?M, PH exhibited 70.823% cell viability and significantly reduced lipid accumulation compared to controls. Gene expression analysis revealed heightened PPAR?, GLUT4, PI3K, FASN expression (P<0.05), and reduced TNF? and IL6 levels. Conclusively, PH upregulates PPAR?, enhancing insulin sensitivity while reducing lipids, TNF?, and IL6. Interpretation & conclusions.: This study investigates the genetic and biochemical effects of Pioglitazone hydrochloride (PH), a PPAR? agonist, on insulin resistance and metabolic disorders in human adipocyte stem cells. PH treatment enhanced insulin sensitivity by upregulating key genes (PPAR?, GLUT4, PI3K, FASN) and reducing inflammatory markers (TNF?, IL6). Cytotoxicity assays confirmed PH's safety at 50 ?M, with significant improvements in lipid accumulation and gene expression. These findings highlight PH's potential as a therapeutic agent for insulin resistance and related complications, warranting further translational studies.
Keywords: Type 2 diabetes mellitus insulin sensitivity, lipid metabolism, adipocyte differentiation, molecular signaling, PPAR? agonist Pioglitazone hydrochloride, semiquantitative PCR and real-time PCR
Edition: Volume 14 Issue 1, January 2025
Pages: 305 - 311
DOI: https://www.doi.org/10.21275/SR25105163035
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