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Research Paper | Biology | Pakistan | Volume 4 Issue 6, June 2015 | Popularity: 6.2 / 10
Establishment of Plant Regeneration Protocol in Cucumber: Fruit Juices Used as Carbon Source
Ikram-ul Haq, Zahra Arshad, Taseer
Abstract: The effect of commercial fruit juices was assessed on callus induction, its proliferation and plant regeneration in cucumber (Cucumis sativus L. ) cv. , Liza. Almost 3-4 mm, cuttings of seedling (7-10 days old) were cultured for callus induction on MS2 [MS0 (Murashige & Skoog, 1962) basal salts, 0.6 mg L-1 2, 4-D (2, 4-Dichlorophenoxy acetic acid), 0.4 mg L-1 BAP (Benzyleaminopurine)] medium and also on simple MS0 medium supplemented with fruit juices in place of 3 % sucrose like as MS2a (MS0 with orange juice), MS2b (apple), MS2c (red grapes), MS2d (strawberry) for callus induction. No callusing was observed on fruits juice cultures while callus proliferation was higher in orange juice culture (MS2a), when 3-weeks old callus sub-cultured from MS2 medium. Maximum plant regeneration (3.980.65 per callus) was observed on *MS2c medium when somatic embryogenesis was induced on MS3 (0.1 mg L-1 BAP, 0.5 mg L-1 glutamate) medium in dark (2-weeks). Root induction efficiency was higher in 1MS2b and 1MS2d 1/2MS) cultures under light condition. After plant hardening, they were transferred to soil conditions.
Keywords: Cucumis sativus L, callus induction, fruit juices, somatic embryogenesis, plant regeneration
Edition: Volume 4 Issue 6, June 2015
Pages: 81 - 84