Development and Validation of the RP-HPLC Method for the Analysis of Chloropyrifos

K. Vinod Kumar, B. Ramachandra, N.V.S.Naidu

Abstract: A simple, selective, precise and accurate High Performance liquid Chromatographic method for the analysis of Chloropyrifos in its formulations was developed and validated in the present study. The mobile phase consist a mixture of Acetonitrile and Potassium Dihydrogen Orthophosphate in the proportion 7030 and adjust the pH to 6.8. This was found to give sharp peak of Chloropyrifos at a retention time of 6.780 min. HPLC analysis of Chloropyrifos was carried out at a wave length of 289 nm with a flow rate of 1.0L/ min. the linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient 0.999 in the concentration range of 12mg to 72mg. The linear regression equation was y = 63577x + 14260. The developed method was employed with a high degree of precision and accuracy for the analysis of Chloropyrifos. The method was validated for accuracy, precision, robustness, ruggedness, specificity. The precision, accuracy, sensitivity, short retention time and composition of the mobile phase indicated that this method is better for the quantification of Chloropyrifos.

Keywords: Chloropyrifos, RP-HPLC Method development, Validation