Analysis of Phylogeny and Evolutionary Divergence of RbcL Sequence of Barleria longiflora L.f

Baskaran, Karthikeyan

Abstract: Barleria longiflora L. f belongs to the family Acanthaceae which is endemic distribution. Presence of this plant in Erattamalai hill, located in Tiruchirappalli District of Tamil Nadu, India. The rbcl gene is widely used in systematic studies to resolve divergences at many taxonomic levels. The collected B. longiflora were subjected to sequence analysis of rbcL gene and the same was deposited in Gene Bank and an accession number has been assigned for the same (Accession No. KR861703.1). The data was further analyzed for the construction of neighbor joining tree and to infer the evolutionary divergence among the maximum identical sequences retrieved from NCBI Gene Bank through BLAST search. The results of the search revealed that the rbcL sequence of B. longiflora has not been sequenced earlier from any part of this world. It is obvious that the rbcL sequence of B. longiflora may be used for the identification of this species reported from any part of the world through BLAST analysis if the identical sequences are submitted to Gene Bank in future.

Keywords: ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit gene, Barleria longiflora, Erattaimalai hill,