Estimation of Cortisone Acetate in Pharmaceutical Anti-inflammatory Drugs by HPLC-UV Technique

H. N. K. AL-Salman, Shaker.A.N.AL-Jadaan

Abstract: The HPLC-UV System was used to assay of Cortisone acetate in Oral Tablets So, compare the bioavailability of two types of Cortisone acetate, commercial formulations and Cortisone acetate analar grade as a test formulation. Cortisone acetate concentrations were analyzed by HPLC-UV System at (=254 nm). The separation was achieved by using the Ion Pac Ercus C18 RP-Column, 5m, 4.6250 mm. The mobile phase consisted of water /acetonitrile (5545). The study of bioequivalence between the two Cortisone acetate formulations were assessed by calculating peaks height. The standard Cortisone acetate eluted at a flow rate of 1.0 ml/min. The method was found to belinear in the range (0.5 to 2.5) g/ml (n = 5) with R2 0.9991, also, the recoveries were range within 99-100 %. The detection limit of quantification (LLOQ) was 0.07909g/ml and lower limit of detection (LLOD) 0.02610 g/ml. showing average intra assay and inter-assay coefficients of RSD % about 0.522 %. The results of recoveries, RSD, and statistical parameters obtained in this study. The accurate, precise and sensitive refers to validation method for determination of Cortisone acetate in anti-inflammatory Pharmaceutical drugs.

Keywords: Cortisone acetate, Oral Tablets, formulation, and HPLC- UV System

How to Cite?: H. N. K. AL-Salman, Shaker.A.N.AL-Jadaan, "Estimation of Cortisone Acetate in Pharmaceutical Anti-inflammatory Drugs by HPLC-UV Technique", Volume 6 Issue 2, February 2017, International Journal of Science and Research (IJSR), Pages: 1178-1183, https://www.ijsr.net/getabstract.php?paperid=ART2017677, DOI: https://dx.doi.org/10.21275/ART2017677