Optimization of Reproducible Biochemical Genotype for Population: The Case of Trifolium Alexandrinum L., Fabacae

S. Rouz, F. Ben Jeddi, M.Zouaghi, Z. Ghrabi-Gammar

Abstract: This study aims to optimize an experimental protocol for giving reproducible genotype of berseem populations. In total, 64 accessions of berseem clover, comprising varieties, landraces and wild forms were used. SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) technique was used and results revealed a high level of polymorphism and a major genetic difference between examined taxa. The optimization of the biochemical characterization revealed that the use of a sample of 200 seeds gives reproducible groups for each population. Efficient maintenance of genetic integrity is obtained when 200 plants are multiplied together and when seeds are collected for each plant. These regeneration strategies are discussed according to the financial and technical context of their application. These results can be used for preservation programs of different populations.

Keywords: Trifolium alexandrinum, berseem, genetic diversity, preservation program