Characterization of Bacteria Found in Soft-Drink through Biochemical Analysis and 16s rDNA PCR

Ashutosh Kumar Mishra, Pushpasingh, Himani Chugh, Brijesh Lohchania, Monica Srivastava

Abstract: Western culture brings us soft drinks as a part of our daily meal. It is aerated beverages which is very easily replacing water from our drinking habits. Ingredients of soft drinks help bacteria to survive in very odd conditions also. Here, efforts are being made to identify if any bacteria is found in our collected samples from open market. To characterize bacteria found we used various techniques like biochemical tests and 16s PCR. We used universal primer to complete PCR. Biochemical tests are the tests used for the identification of bacteria species based on the differences in the biochemical activities.16sr PCR is a technique to identify conserved sequence of bacteria.16sr PCR gel electrophoresis image is analyzed using TotalLab Quant version 12.4 software. This software analyses gel image with respect to ladder run along with samples and generates reports in term of molecular weight of each electrophoresis band.

Keywords: 16s rDNA, Biochemical test, Total Lab Quant, Soft drink, Universal primer