Biogenic Synthesis of Silver Nanoparticles using Leaves of Crinum asiaticum Linn.

Malathi. R, Ganesan. V

Abstract: In this study, we report the phytofabrication of silver nanoparticles using the leaf broth of Crinum asiaticum Linn. (Family: Amaryllidaceae). The leaf broth was added to aqueous solution of silver nitrate and it is known as reaction medium. This reaction medium was kept in an incubator cum shaker with 250rpm at 270c for 24 hours to reduce the silver nitrate into silver nanoparticles. The reaction medium changed its colour from pale yellow to dark brown observed the incubation period. It indicates the formation of silver nanoparticles. The synthesized silver nanoparticles were characterized by UV-Visble spectroscopy, X-ray diffraction patterns (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) with Energy Dispersive X-ray (EDX) patterns and Transmission Electron Microscopy (TEM). This type of phyto-mediated synthesis appears to be cost effective, eco-friendly and an alternative to conventional physical and chemical methods for the synthesis of silver nanoparticles.

Keywords: Crinum asiaticum, Leaf broth, Silver nanoparticles, Reaction medium