Physico-Chemical Parameters and Hptlc Qualitative Analysis of Leaf Extracts of syzygium Cumini

Bhagwan Singh, Rajasekaran .S

Abstract: Objective: This study aimed to evaluate the physico-chemical parameters and hptlc qualitative analysis of leaf extracts of syzygiumcumini. Methods: The shade dried parts of the plant leafs were subjected for the study of physico-chemical parameters and accurate HPTLC method by using Rutin, Gallic acid and quercetin as a standard marker compound with mobile phase of Toluene-Ethyl Acetate-Formic acidMethanol (3: 6: 1.6: 0.4). The detection of Rutin, Gallic acid and quercetin were performed at 254 nm respective. Results: The effect of Syzygiumcumini having significant foreign matter2%, Moisture content3%, Total ash5-6%, Acid insoluble ash0.85%, Ethanol soluble extractive value1.45% and Water soluble extractive value1.35%. The detection of Rutin, Gallic acid and quercetin were performed at 254 nm respectively. Conclusion: It is concluded from this study of physico-chemical parameters and hptlc qualitative analysis of syzygiumcumini possess significant effects.

Keywords: Syzygiumcumini, physico-chemical parameters, hptlc, Rutin, Gallic acid and quercetin