Comparative Study of Conventional Staining Techniques in Cytology

Faris Merghani A Elmahdi, Thabat Ibrahim Alrfaie, Ehdaa Mahmmoud Alsubaihi, Bayan Abdurahman Alhakami, Ghazal Mahmoud Shalabi, Bushra Anwar Kurdi, Abrar Khalid Aloufi, Saad Ali S. Aljohani

Abstract: Background: Exfoliative cytology is the histopathologic examination of cells that have been obtained by their physical removal, followed by their placement on a glass slide, and then appropriately stained [1].The aim of this study was to compare the staining of cytologic Papanicolaou stain, hematoxylin andeosin stain, and may-grunwald-giemsa stain in oral mucosa smear. Materials and Methods: A total of 150 Cytological oral smears were taken from 50 volunteers using a sterile wooden tongue depressor. In each case, the surface epithelium of the buccal mucosa was scraped and applied to a clean frosted glass slide. The scraped cells were placed onto pre-cleaned slides. three slides were made from each subject. One was air-dried and stained with the (MGG) stain, while the other was wet fixed and stained with PAP and H&E. Results: The nuclear structure was excellent stained with Pap and H&E and was poor with MMG representing 88%, 80, and 5% respectively while the cytoplasm and background were excellent stained with Giemsa representing 80% and 60% and respectively. Conclusion: Pap-stained smears were better than H&E and Grunwald- Giemsa stain for oral cytology.

Keywords: Comparative, Staining Techniques, Cytology

How to Cite?: Faris Merghani A Elmahdi, Thabat Ibrahim Alrfaie, Ehdaa Mahmmoud Alsubaihi, Bayan Abdurahman Alhakami, Ghazal Mahmoud Shalabi, Bushra Anwar Kurdi, Abrar Khalid Aloufi, Saad Ali S. Aljohani, "Comparative Study of Conventional Staining Techniques in Cytology", Volume 11 Issue 5, May 2022, International Journal of Science and Research (IJSR), Pages: 876-879, https://www.ijsr.net/getabstract.php?paperid=SR22514114506, DOI: https://dx.doi.org/10.21275/SR22514114506