Quantitative Phytochemical Analysis and DNA Barcoding Studies of Artocarpusheterophyllus

Charishma C Gowda, Dr. Shruthi SD

Abstract: Artocarpusheterophyllus, commonly known as jackfruit, is a healthful source that contains various phytochemicals, vitamin C, copper, potassium, manganese, magnesium and riboflavin. This fruit improves immunity and thus have desirable pharmacological activities such as anti - inflammatory, anti - diabetic, anti - angiogenic, anti - oxidant, anti - cancerous, anti - cholesterolemic and wound healing applications. The objectives of the study include performing DNA Barcoding studies, anti - ulcer activity and to estimate various phytochemicals in jackfruit leaves. DNA barcoding technique was conducted to authenticate the jackfruit species. The total phenolic content and the flavonoids were estimated by plotting gallic acid and quercetin standard curve. The antiulcer activity was tested by performing antibacterial test using well - diffusion method where in the zone of inhibition was measured. The ratio of A260/A280 was found to be 16.667 and the concentration of DNA was found to be 3?g/ml. Various phytochemicals were analysed including total phenolic content (484?0.15 ?g/ml), flavonoid content (1996.3?0.02 ?g/ml), ursolic acid (23?0.02 ?g/ml), saponins, triterpenoids and steroids. The zone of inhibition was found in higher scale with Pseudomonas aeruginosa than Staphylococcus aureus. From the results, it was evident that the leaf extract possessed high activity against gram - negative bacteria in comparison with gram - positive bacteria.

Keywords: flavanoids, pharmacological activities, phytochemicals, well - diffusion, ursolicacid