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Research Paper | Histopathology | Saudi Arabia | Volume 13 Issue 7, July 2024 | Rating: 5.6 / 10
Identification of Mucopolysaccharide Types in Diabetic Placenta and Umbilical Cord Tissues
Faris M. Elmahdi [2] | Lobna E Ali | Sahar E. Osman | Hala G. Alssied | Lina A. Salim
Abstract: Background: The health of the placenta significantly influences the fetus's well - being. Placental abnormalities, especially in diabetic pregnancies, can lead to various complications affecting both the mother and the fetus. Understanding the types and distribution of mucopolysaccharides in the placenta and umbilical cord tissues of diabetic pregnancies is crucial for improving diagnostic and therapeutic strategies. Objective: The aim is to identify the types of mucopolysaccharides present in the diabetic placenta and the tissues of the umbilical cord. Materials and Methods: We conducted this non - interventional descriptive study. We analyzed a total of 36 biopsies, comprising 18 from diabetic pregnancies and 18 from healthy pregnancies. We prepared sections (3 - 5 microns) from these tissues and processed them using various staining techniques, such as hematoxylin and eosin (H&E), Alcian Blue - PAS combination, and mild methylation with Alcian Blue staining. The data were analyzed using SPSS. Results: From 216 samples, sections of the umbilical cord stained with H&E showed 72% normal histological structures. Combination techniques revealed that 34% of mucopolysaccharides were neutral and strongly acidic. Mild methylation revealed that 45% of mucopolysaccharides were strongly carboxylated and 33% were strongly sulfated. Diabetic placenta sections stained with H&E showed 33% inflammatory changes, with basement membrane thickening. Combination techniques were used to detect 45% neutral and strongly acidic mucopolysaccharides. Mild methylation revealed that 39% had no acidic mucopolysaccharides, 33% were strongly sulfated, and 17% were strongly carboxylated. Conclusion: The study highlights significant differences in the types of mucopolysaccharides between diabetic and non - diabetic placental and umbilical cord tissues. Diabetic tissues exhibited higher levels of strongly carboxylated and sulfated mucopolysaccharides, indicating potential markers for diabetes complications during pregnancy.
Keywords: Placenta, Umbilical Cord, Mucopolysaccharides, Diabetes
Edition: Volume 13 Issue 7, July 2024,
Pages: 952 - 957